Exploring the Binding and ATPase Activity of Msh2-Msh6 with Four Way Junction DNA
Abstract: Msh2-Msh6 is a eukaryotic ATP-dependent mismatch repair (MMR) protein that plays a key role in maintaining the fidelity of the genetic code. The protein recognizes and initiates repair for single base pair mismatches and short insertion/deletion loops (IDL). Fluorescence in solution assays have revealed that Msh2-Msh6 binds with the highest affinity to G:T mismatch … Read more