Elucidation of Msh4-Msh5’s Binding and Hydrolysis of ATP via the Creation of ATPase mutants and Protein Purification using the Maltose Binding Protein
Abstract: Msh4-Msh5 or MutSy is an important protein heterodimer that belongs to the family of MutS repair proteins that are involved in post-replicative mismatch repair and help to maintain genome integrity and cellular stability. Msh4-Msh5 is critical for stabilizing joint molecule DNA recombination intermediates, for promoting crossover recombination, and for proper assembly of the synaptonemal complex. … Read more